{"id":339,"date":"2012-11-02T10:10:09","date_gmt":"2012-11-02T10:10:09","guid":{"rendered":"http:\/\/towardsdolly.wordpress.com\/?p=339"},"modified":"2012-11-02T10:10:09","modified_gmt":"2012-11-02T10:10:09","slug":"chicken-ifg-i-cdna-molecular-cloning-and-gene-sequence-analysis","status":"publish","type":"post","link":"https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/2012\/11\/02\/chicken-ifg-i-cdna-molecular-cloning-and-gene-sequence-analysis\/","title":{"rendered":"Chicken IGF-I cDNA \u2013 Molecular Cloning and Gene Sequence Analysis"},"content":{"rendered":"<p>Some of the most interesting papers I\u2019ve found so far in my cataloguing of the Institute of Animal Physiology and Genetics Research (IAPGR) have been the ones on gene sequencing, molecular cloning and analysis. This is the first paper I&#8217;ve come across so far that shows an animal gene sequence next to a<a href=\"http:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/files\/2012\/11\/chickenigfsequenceimage2.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-341 alignright\" title=\"ChickenIGFSequenceimage\" alt=\"\" src=\"http:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/files\/2012\/11\/chickenigfsequenceimage2.jpg?w=243\" height=\"300\" width=\"243\" srcset=\"https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/files\/2012\/11\/chickenigfsequenceimage2.jpg 2656w, https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/files\/2012\/11\/chickenigfsequenceimage2-243x300.jpg 243w, https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/files\/2012\/11\/chickenigfsequenceimage2-831x1024.jpg 831w, https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/files\/2012\/11\/chickenigfsequenceimage2-624x768.jpg 624w\" sizes=\"(max-width: 243px) 100vw, 243px\" \/><\/a> human one.<\/p>\n<p>This image of a genomic sequence (Figure 3) in D. H. Fawcett and G. Bulfield\u2019s article, \u2018Molecular cloning, sequence analysis and expression of putative chicken insulin-like growth factor-I cDNAs\u2019 in the <i>Journal of Endocrinology <\/i>(1990), 4, 201-211 shows the \u2018potential splice donor sites at the excon 2 (5\u2019) intron boundary in the chicken compared with the corresponding human sequence and the chicken and human cDNAs.\u2019<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Some of the most interesting papers I\u2019ve found so far in my cataloguing of the Institute of Animal Physiology and Genetics Research (IAPGR) have been the ones on gene sequencing, molecular cloning and analysis. This is the first paper I&#8217;ve &hellip; <a href=\"https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/2012\/11\/02\/chicken-ifg-i-cdna-molecular-cloning-and-gene-sequence-analysis\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":31,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"jetpack_publicize_message":"","jetpack_is_tweetstorm":false,"jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false}}},"categories":[3],"tags":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_shortlink":"https:\/\/wp.me\/p40Aqf-5t","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/wp-json\/wp\/v2\/posts\/339"}],"collection":[{"href":"https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/wp-json\/wp\/v2\/comments?post=339"}],"version-history":[{"count":0,"href":"https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/wp-json\/wp\/v2\/posts\/339\/revisions"}],"wp:attachment":[{"href":"https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/wp-json\/wp\/v2\/media?parent=339"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/wp-json\/wp\/v2\/categories?post=339"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/libraryblogs.is.ed.ac.uk\/towardsdolly\/wp-json\/wp\/v2\/tags?post=339"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}